A highly efficient urea detection using flower-like zinc oxide nanostructures
By Manvi Tak and Vinay Gupta and Monika Tomar
Published in Materials Science and Engineering: C
2015
Abstract
A novel matrix based on flower-like zinc oxide nanostructures (ZnONF) has been fabricated using hydrothermal method and exploited successfully for the development of urea biosensor. Urease (Urs) is physically immobilized onto the ZnO nanostructure matrix synthesized over platinized silicon substrate. The surface morphology and crystallographic structure of the as-grown ZnONF have been characterized using a scanning electron microscope (SEM) and X-ray diffraction (XRD) techniques. The fabricated amperometric biosensor (Urs/ZnONF/Pt/Ti/Si) exhibits a linear sensing response towards urea over the concentration range 1.65 mM to 16.50 mM with an enhanced sensitivity (~ 132 ?A/mM/cm2) and a fast response time of 4 s. The relatively low value of Michaelis