Selenium containing conducting polymer based pyranose oxidase biosensor for glucose detection
By Tugba Ceren Gokoglan and Saniye Soylemez and Melis Kesik and Sinem Toksabay and Levent Toppare
Published in Food Chemistry
2015
Abstract
A novel amperometric pyranose oxidase (PyOx) biosensor based on a selenium containing conducting polymer has been developed for the glucose detection. For this purpose, a conducting polymer; poly(4,7-bis(thieno[3,2-b]thiophen-2-yl)benzo[c][1,2,5] selenadiazole) (poly(BSeTT)) was synthesized via electropolymerisation on gold electrode to examine its matrix property for glucose detection. For this purpose, PyOx was used as the model enzyme and immobilised via physical adsorption technique. Amperometric detection of consumed oxygen was monitored at ?0.7 V vs Ag reference electrode in a phosphate buffer (50 mM, pH 7.0). KMapp, Imax, \LOD\ and sensitivity were calculated as 0.229 mM, 42.37 nA, 3.3