Sequence-Specific Electrical Purification of Nucleic Acids with Nanoporous Gold Electrodes
By Daggumati, Pallavi; Appelt, Sandra; Matharu, Zimple; Marco, Maria L.; Seker, Erkin
Published in Journal of the American Chemical Society
2016
Abstract
Nucleic-acid-based biosensors have enabled rapid and sensitive detection of pathogenic targets; however, these devices often require purified nucleic acids for analysis since the constituents of complex biological fluids adversely affect sensor performance. This purification step is typically performed outside the device, thereby increasing sample-to-answer time and introducing contaminants. We report a novel approach using a multifunctional matrix, nanoporous gold (np-Au), which enables both detection of specific target sequences in a complex biological sample and their subsequent purification. The np-Au electrodes modified with 26-mer DNA probes (via thiol