High current, low redox potential mediatorless bioanode based on gold nanoparticles and glucose dehydrogenase from Ewingella americana
By D. Ratautas and A. Lauryn?nas and M. Dagys and L. Marcinkevi?ien? and R. Me
Published in Electrochimica Acta
2016
Abstract
The action of bacterial glucose dehydrogenase (GDH) from Ewingella americana on the gold nanoparticles (AuNPs) modified with 4-aminothiophenol (4-ATP) and 4-mercaptobenzoic acid (4-MBA) was studied. \GDH\ adsorbed on these nanostructured electrodes catalysed glucose oxidation in a mediator-free manner. The voltammetric investigations suggested the direct electron transfer between the enzyme active centre and the electrode. The maximum biocatalytic current of 10 mA cm?2 and low onset potential (