A highly sensitive amperometric immunosensor probe based on gold nanoparticle functionalized poly (3, 4-ethylenedioxythiophene) doped with graphene oxide for efficient detection of aflatoxin {B1}
By Sharma, Arati; Kumar, A.; Khan, Raju
Published in Synthetic Metals
2018
Abstract
In the present work, a platform of Poly (3, 4-ethylenedioxythiophene) (PEDOT) and graphene oxide (GO) composite decorated with spherical gold nanoparticles (AuNPs) has been developed for rapid electrochemical detection of aflatoxin {B1} (AFB1). Electrochemical deposition of {EDOT} onto glassy carbon electrode (GCE) keeping {GO} as dopant followed by introduction of AuNPs has been achieved resulting in nanohybrid AuNPs/PEDOT-GO electrode. The antibody anti-aflatoxin {B1} (anti-AFB1) has been further covalently immobilized onto the surface of AuNPs/PEDOT-GO using EDC/NHS coupling. The morphological and surface characteristics have been studied using Field Emission Scanning Microscopy (FESEM) and contact angle measurements. Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) studies have been carried out to investigate the electro-catalytic behavior of the modified electrodes. The heterogeneous rate constant (ks) and transfer coefficient (?) have been determined by using Laviron